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Background: SLE is treated currently by multiple immunosuppression, but side-effects are obvious after long time administration. This study was to observe changes in T, B cells, NK cells and IL12 in patients with systemic lupus erythematosus (SLE) before and after treatment of eliminating intermittent white blood cells and to probe the mechanism of this treatment.
Methods: In 23 patients with SLE, 5 were male and 18 female, with an average age of 15.78¡À5.40 years. These patients accepted treatment of eliminating intermittent white blood cells. The expressions of CD19+, CD3+, CD4+CD8+, CD4+/CD8+, CD(15+56)+ were tested by flow cytometry before and after the treatment. The amounts of immunoglobulins, IgM, IgG, IgA in periphery blood were measured separately by immunoradiometric analysis before and after the treatment. The level of IL12 was detected by ELISA. Twenty volunteers served as controls.
Results: The expression of CD19+ in the patients increased markedly before the treatment. Statistical significance was noted in the control group and the patient group after the treatment (P<0.01 or P<0.001). The expression of IgM and IgG increased markedly before the treatment. Statistical significance was seen between the control group and the patient group after the treatment (P<0.01 or P<0.001). The expressions of activated CD3+ and CD8+ increased markedly in the patients with SLE before the treatment (P<0.05) (P<0.01 or 0.001) respectively. The expressions of CD3+ and CD4+ decreased markedly (P<0.01 or P<0.05) (P<0.001) respectively. The ratio of CD4+ to CD8+ decreased markedly (P<0.01). The expression of CD3+ after the treatment decreased more remarkably in the patients with SLE than in the control group (P<0.05). The changes in the expression of CD(15+56)+ suggested that the expression of CD(15+56)+ increased markedly before the treatment. Significant statistical difference was observed in the patient group and the control group after the treatment (P<0.05 or P<0.001). The expression of IL12 in the patients with SLE decreased, but it decreased more significantly than in the control group before the treatment (P<0.05 or P<0.01). Conclusions: Since patients with SLE have the disturbances in T, B cellular immunology and NK cells, IL12, the treatment of eliminating intermittent white blood cells has regulatory effects on T, B cells immunology and NK cells, IL12 in the patients with SLE.
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